The Vipers ended their great run in the Primary cup losing 4-2 to Tacket Street Old Boys in the last eight.
Ashley Brame and Jutty Nunn netted for the Vipers.
In Division One, Home Guards visitors at Gainsborough Road were Westbury United, where the spoils were shared in a 3-3 draw.
Jez Knight, Adam Cornish and Freddie Hughes netted for United, while replying for Guard were Jamie Barclay, Matt Creaton (penalty) and Callum Brown.
St Edmunds 1965 entertained Bury Town Rams A at the Heath with the Saints running out 7-1 winners.
Hitting the net for St Edmunds were Owen Manning (3), Ross Whiting (2), Ben Tooke and Sam Deeming.
Dave Venables scored for the Ram.
In Division Two, Trunk moved into contention for the top spot with a 3-1 home win against Bury Wanderers, where Mica Cummins (2) and Craig Defew were on target.
Jamie Maxwell scored for Wanderers.
In the Bill Utting Cup, Third Division AFC Weeting’s opponents were Division One side Wortham.
Weeting booked their semi-final spot with a 3-2 home win thanks to goals from Paul Carr, Kurt Baker and Daniel Malster.
Tom Mayhew and Reece Monro netted for Wortham
In an all Division Two cup-tie Bury Town Rams B side entertained Thurston at Northumberland Avenue.
Thurston won by the goal in seven thanks to efforts from Martin Bennet, Declan Smith and Jamie Young (2).
Nathan Head and Matt Hawkins scored for Ram B.
At the Village Hall ground, Stanton Reserves (Division One) entertained Division Three top side Walsham le Willows.
Stanton sealed a 3-0 victory with goals from Max Milton (2) and Ben Mayhew.
At Hepworth, Wortham Reserves (Division Three) entertained RF Saints (Division Two).
With the scores level at 3-3 after 90 minutes the resulting penalty kicks went the way of the Saints 3-2.
Goalsscorers in normal time for the Saints were Lee Sayer (2) and Luke Frammingham.
Casey Warnes (2) and Jay Rose were the Wortham scorers.