Greene King in Bury St Edmunds has raised £300,000 for Macmillan Cancer Support, in its first year of a three-year partnership.
The brewery and pubs firm is committed to raising £1m for the charity over the three years.
Among its fund-raising activities, it brewed a summer guest ale Caritas (Latin for charity), with 5p for every pint sold going to Macmillan.
Rooney Anand, Greene King chief executive, said: “We have taken Macmillan to heart.”
The money come from a variety of events and activities in the company’s pubs, breweries, hotels, restaurants, depots and head office.
Fund-raising activities have included cake baking, marathon and half marathon runs, bike rides, swim-a-thons, walking and sky diving.
“This great cause has united our employees and our customers and we have been overwhelmed by the goodwill and the enthusiasm for the partnership from every corner of our business,” said Mr Anand.
The charity provides both emotional, financial, practical and medical support to cancer patients and their families.
Lynda Thomas, director of fund-raising at Macmillan Cancer Support, said: “Raising £300,000 is a fantastic achievement, and will go a long way towards helping Macmillan support people affected by cancer, ensuring that no one has to face cancer alone.”